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quantikine elisa kit  (R&D Systems)


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    Structured Review

    R&D Systems quantikine elisa kit
    Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+elisa+kit/Human+M-CSF+Quantikine+ELISA+Kit/pm42821460-65-17-22
    Average 94 stars, based on 68 article reviews
    quantikine elisa kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Lower airway microbiota compositions and diversity among ventilator-associated pneumonia patients across COVID-19 epidemic phases: a retrospective study.
    Article Snippet: .. Serum cytokines, including tumor necrosis factor-ɑ (TNF-ɑ), interleukin-1β (IL-1β), interleukin-2 receptor (IL-2R), interleukin-6 (IL-6), interleukin-8 (IL-8), and interleukin-10 (IL-10), were quantified by enzyme-linked immunosorbent assay (ELISA) using commercially available kits (Human ELISA Kit, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. ..

    Article Title: Prognostic Analysis of VEGF, CRP and Contrast-Enhanced Ultrasound Combined with Interventional Embolization for Primary Liver Cancer.
    Article Snippet: .. VEGF was assessed using a human ELISA kit (R&D Systems, Quantikine, Cat.DVE00), with a detection limit of 9 pg/mL, a linearity of 31.2-2000 pg/mL, repeated Wells, and 4PL fitting. .. CRP was assessed by immunoturbidimetry (Roche CRP Latex, Cat.05172373190, c702 platform, linear 0.3-350 mg/L).

    Article Title: Cord Blood Exosomal miRNAs from Small-for-Gestational-Age Newborns: Association with Measures of Postnatal Catch-Up Growth and Insulin Resistance.
    Article Snippet: .. Circulating HMW-adip was measured using a specific human ELISA kit (R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Cord Blood Exosomal miRNAs from Small-for-Gestational-Age Newborns: Association with Measures of Postnatal Catch-Up Growth and Insulin Resistance
    Article Snippet: .. Circulating HMW-adip was measured using a specific human ELISA kit (R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Year-round supplementation of direct-fed microbials to cow-calf pairs and its effects on maternal and offspring productive performance
    Article Snippet: 330 Serum concentrations of IgG were assessed using a bovine-specific ELISA kit (E11-118; 331 Bethyl Laboratories, Inc., Montgomery, TX). .. Plasma concentrations of IGF-1 were assessed using 332 a commercial human ELISA kit (SG100B; R&D Systems, Inc., Minneapolis, MN, USA), 333 previously validated for bovine samples (Moriel et al., 2012). .. Quantitative colorimetric kits were 334 used to determine the plasma concentrations of glucose and urea N (G7521 and B7551, 335 respectively; Pointe Scientific Inc.; Moriel et al., 2015), and NEFA (HR Series NEFA-2; Wako 336 Pure Chemical Industries Ltd, Richmond, VA; Palmer et al., 2022).

    Article Title: Lower airway microbiota compositions and diversity among ventilator-associated pneumonia patients across COVID-19 epidemic phases: a retrospective study
    Article Snippet: .. Serum cytokines, including tumor necrosis factor-ɑ (TNF-ɑ), interleukin-1β (IL-1β), interleukin-2 receptor (IL-2R), interleukin-6 (IL-6), interleukin-8 (IL-8), and interleukin-10 (IL-10), were quantified by enzyme-linked immunosorbent assay (ELISA) using commercially available kits (Human ELISA Kit, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. ..

    Article Title: Three-Year Follow-Up of Neoadjuvant Tislelizumab with Chemotherapy in Locally Advanced Gastric and Gastroesophageal Junction Adenocarcinoma: Revealing Cancer-Associated Fibroblast Heterogeneity Corresponding to PD-1 Blockade Efficacy.
    Article Snippet: .. IFN-γ and IFN-α, IL-16 Enzyme-Linked Immunosorbent Assay (ELISA): The concentration of IFN-γ and IFN-α, IL-16 in culture supernatants was determined using a Human ELISA Kit (R&D Systems) according to the manufacturer’s protocol. ..

    Article Title: Prognostic Analysis of VEGF, CRP and Contrast-Enhanced Ultrasound Combined with Interventional Embolization for Primary Liver Cancer
    Article Snippet: .. VEGF was assessed using a human ELISA kit (R&D Systems, Quantikine, Cat.DVE00), with a detection limit of 9 pg/mL, a linearity of 31.2-2000 pg/mL, repeated Wells, and 4PL fitting. .. CRP was assessed by immunoturbidimetry (Roche CRP Latex, Cat.05172373190, c702 platform, linear 0.3-350 mg/L).

    Clinical Proteomics:

    Article Title: Year-round supplementation of direct-fed microbials to cow-calf pairs and its effects on maternal and offspring productive performance
    Article Snippet: 330 Serum concentrations of IgG were assessed using a bovine-specific ELISA kit (E11-118; 331 Bethyl Laboratories, Inc., Montgomery, TX). .. Plasma concentrations of IGF-1 were assessed using 332 a commercial human ELISA kit (SG100B; R&D Systems, Inc., Minneapolis, MN, USA), 333 previously validated for bovine samples (Moriel et al., 2012). .. Quantitative colorimetric kits were 334 used to determine the plasma concentrations of glucose and urea N (G7521 and B7551, 335 respectively; Pointe Scientific Inc.; Moriel et al., 2015), and NEFA (HR Series NEFA-2; Wako 336 Pure Chemical Industries Ltd, Richmond, VA; Palmer et al., 2022).

    Concentration Assay:

    Article Title: Three-Year Follow-Up of Neoadjuvant Tislelizumab with Chemotherapy in Locally Advanced Gastric and Gastroesophageal Junction Adenocarcinoma: Revealing Cancer-Associated Fibroblast Heterogeneity Corresponding to PD-1 Blockade Efficacy.
    Article Snippet: .. IFN-γ and IFN-α, IL-16 Enzyme-Linked Immunosorbent Assay (ELISA): The concentration of IFN-γ and IFN-α, IL-16 in culture supernatants was determined using a Human ELISA Kit (R&D Systems) according to the manufacturer’s protocol. ..



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    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by <t>DuoSet‐ELISA</t> kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.
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    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by <t>DuoSet‐ELISA</t> kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.
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    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by <t>DuoSet‐ELISA</t> kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.
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    Image Search Results


    Journal: Advances in Pharmacological and Pharmaceutical Sciences

    Article Title: Evaluation of Biological Effect of Triticum vulgare Extract Compared With Hyaluronic Acid in In Vitro Tissue Models

    doi: 10.1155/adpp/8857215

    Figure Lengend Snippet: Primer sequences used for qRT‐PCR.

    Article Snippet: Supernatants of scratched HaCaT cells (treated and untreated) and supernatants of 3D skin previously treated with LPS and after being scratched were collected after 48 and 72 h, respectively, and analyzed using ELISA for quantification of HBD2 production (Elabscience) according to the manufacturer’s instructions.

    Techniques:

    Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by DuoSet‐ELISA kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.

    Journal: Chembiochem

    Article Title: Functional Heterogeneity of the Autism Spectrum Disorder‐Associated Gut Microbial Ecosystem Revealed by Fecal Lipopolysaccharides and Bacterial Proteomic Profiling

    doi: 10.1002/cbic.70557

    Figure Lengend Snippet: Effects of fecal LPS on HMC3 microglia cell viability and activation. (A) Bright‐field image of HMC3 cells (40× magnification, scale bar: 20 µm). (B) HMC3 microglia cell viability after 24 h exposure to increasing concentrations (0.1, 1, 10, 100 ng/mL) of the pooled fecal LPS preparations obtained from the ASD and NASD cohorts. Cell viability was expressed as % relative to non‐stimulated (NS) cells and assessed by MTT assay (570 nm). NS cells were also considered as negative control. (C,D) Quantification of (C) IL‐8 and (D) IL‐6 levels following LPSs treatment, measured by DuoSet‐ELISA kit. Three independent experiments ( n = 3) were performed, with each experimental condition tested in triplicate wells within each experiment. Statistical significance was assessed by ordinary one‐way ANOVA followed by Tukey's multiple‐comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus NS; # p < 0.05, ### p < 0.001, #### p < 0.0001, versus LPS.

    Article Snippet: Cytokine levels (pg/mL) in HMC3 supernatants were measured using DuoSet ELISA kits (R&D Systems, Minneapolis, MN, USA) for human IL‐8 (#DY208) and IL‐6 detection (#DY206).

    Techniques: Activation Assay, MTT Assay, Negative Control, Enzyme-linked Immunosorbent Assay, Comparison